Among the different mammalian isoforms of Carbonic Anhydrase, the hCA VII is mainly expressed in the brain where it is involved in several neurological diseases. Thereby hCA VII has been validated as an attractive target for the discovery of selective inhibitors for the treatment of epilepsy and neurological pain. To identify new chemical entities as carbonic anhydrase inhibitors (CAIs) targeting hCA VII, we used a structure-based approach. By means of LigandScout software we built pharmacophore models from crystal structures of two well-known CAIs in complex with hCA VII. A merged pharmacophore hypothesis has been obtained. Subsequently, a focused library of compounds was screened against pharmacophore model and the most interesting hits were docked into the crystal structure of hCA VII. As a result, we identified new compounds displaying significant CA inhibitory effects in the nanomolar range.
Keywords: CAIs; CAs; Carbonic anhydrase; Docking; H; H-bond acceptors; H-bond donors; HBA; HBD; LigandScout; Structure-based pharmacophore model; Virtual screening; carbonic anhydrase inhibitors; carbonic anhydrases; hCA VII; hydrophobic group.
Copyright © 2013 Elsevier Masson SAS. All rights reserved.